The 630nm CNI laser being used in photo dynamic therapy. Antimicrobial photodynamic therapy (aPDT) leads to the generation of reactive oxygen species (ROS) that destroys bacterial cells in presence of a photosensitizer, visible light, and oxygen.

Aligarh Muslim University has taken Enterococcus faEcalis and Streptococcus mutans as monospecies culture and their dualspecies culture biofilm. Antibacterial effect was evaluated by colony forming unit while antibiofilm action by crystal violet and congored binding assays. Finally they found that reactive oxygen species and singlet oxygen yield was found to be light dose dependent and antimicrobial photodynamic efficiency is directly related to the ROS production. They use a 630nm CNI laser to complete the experiement and published their paper in Photodiagnosis and Photodynamic Therapy, 2019 – Elsevier.