532nm single frequency lasers used in Optical Diffraction Tomography.

Korea Advanced Institute of Science and Technology presents a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM). A digital micromirror device is utilized to generate structured illumination patterns for both ODT and SIM, which enables fast and stable measurements. They use CNI 473nm and 532nm single frequency lasers to complete the experiment, and the paper of this research is published in Scientific Reports.